Services and description

PRICE (€)

ISOLATION AND PURIFICATION

Isolation from a mixed culture plate

50

Plate counts in general media

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PRESERVATION

Freeze-dried (minimum order 8 ampoules per strain)

130

Biological material custody (in -80ºC)

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BACTERIAL SUPPLY

Active growing culture on request (from frozen culture at -80ºC)

70

Actively growing culture on request (from freeze dried)

200

Freeze dried ampoule

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BACTERIAL IDENTIFICATION

Partial sequencing of 16S rRNA gene

100

Nearly complete sequencing of 16S rRNA gene (∼ 1400 bp)

200

Sequence analysis of housekeeping genes or any specific gene

100

PCR amplicon (presence/absence of specific genes)

30

Phylogenetic analyses (single gene or multiple genes)

350

MALDI-TOF MS

35

Genomic DNA extraction (25 µl)

110

Whole Genome Sequencing and Analysis (1 prokaryotic strain; includes assembly, annotation, checking by 16S rRNA gene sequencing, and genomic comparisons)

500

BACTERIAL CHARACTERIZATION

Morphological characterization (cell and colony morphology/size, Gram, KOH, catalase, oxidase)

50

Metabolic activities (e.g. API® strips)

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Pulsed-Field Gel Electrophoresis (PFGE)

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Multi-locus Sequence Typing (MLST)

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Fourier-Transform Infrared (FT-IR) spectroscopy typing

30

Plasmid (DNA extraction, replicon typing)

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Gene location (chromosomal/plasmid)

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Antibiotic Susceptibility Testing (according to CLSI/EUCAST guidelines)

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Molecular characterization of antimicrobial resistance mechanisms (PCR and/or sequencing)

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Molecular characterization of virulence mechanisms (PCR and sequencing)

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Molecular characterization of bacteriocins (PCR and sequencing)

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Carbapenemases production (Blue-Carba)

5

In vitro biofilm production assays

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Genetic transfer (conjugation, transformation)

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MICROBIOME CHARACTERIZATION

Culturomic (urine, water, food)

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16S rRNA amplicon sequencing

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Shotgun metagenomic sequencing

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DEPOSIT OF BACTERIAL STRAINS

Public deposit

Free

Safe deposit (1 yr of storage)

250

Renew the safe deposit service

80

Release of a sample of a safe deposit culture

50

OTHER SERVICES

Consultancy (e.g., safety potential of microorganisms according to purpose of use – food and pharmaceutical industry)

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Training:

*Course on preservation and quality control of microbial strains

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*Course on bacterial taxonomy (genomic comparisons and phenotypic features)

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